Federal Labor has again called into question Speaker Bronwyn Bishop’s impartiality over allegations she used her parliamentary suite for fundraising purposes.

Mrs Bishop reportedly hosted a Liberal Party fundraising event in her suite on the night of the May 13 budget.

Labor says if the claim is proved, Mrs Bishop should stand down.

“If this is true, her position as Speaker is no longer tenable,” manager of opposition business Tony Burke told AAP in a statement.

The Speaker’s office was meant to be owned by the parliament.

“It’s not a venue for hire,” Mr Burke said.

But a spokesman for Mrs Bishop said she had not broken any electoral laws by holding the fundraising dinner.

No taxpayer funds were used for the event and all costs associated with Mrs Bishop’s private functions are charged to her private account.

“The Speaker does from time to time have private functions at parliament, as do many other members and senators,” the spokesman told News Corp.

Labor has previously questioned Mrs Bishop’s “serious partiality” in her question time rulings, unsuccessfully moving a motion of no confidence in her in March.