Despite being married to Hugh Hefner for the last five years of his life, his widow Crystal Harris isn’t in line to inherit any of his estimated $US110 million fortune, according to reports.

On Wednesday, the 91-year-old Playboy founder “passed away from natural causes at his home, surrounded by loved ones,” a rep told People magazine.

Harris is thought to have been among those loved ones, but reports claim an “iron-clad prenuptial agreement” meant the former Playmate of the Year was never added to Hefner’s will.

Us Weekly reported in 2013 that the publisher’s fortune would be divided among his four adult children, the University of Southern California and various charities.

Despite Harris, 31, not having been inked in as a recipient, she will be “looked after,” said the magazine.

As police lined the driveway of the Playboy Mansion early Thursday morning, two hearses left the famous residence.

Hefner will be buried in the Westwood Village Memorial Park in Los Angeles next to actress Marilyn Monroe, who graced the cover of the first-ever Playboy in 1953.

He bought the plot in 1992 for $US75,000.

“Jay Leno suggested that if I was going to spend that kind of money, I should actually be on top of her,” Hefner said in a 2000 Playboy interview.

“But to me there’s something rather poetic in the fact that we’ll be buried in the same place.”

Harris was unsighted as her husband left his beloved home for the last time, but during Hefner’s life she was one of his most visible lady loves.

A model, she first met Hefner when she showed up in a French maid outfit for a Halloween party at the Playboy Mansion in 2008.

At the time, Hefner was dating and living with seven women, including three of his Girls Next Door, Holly Madison, Kendra Wilkinson and Bridget Marquardt.

He eventually took up with Harris and on Christmas Eve 2010, Hefner popped the question, hiding a ring inside a Little Mermaid music box.

But five days before the planned 2011 wedding, the 300 invited guests were told the bride had called things off.

“Multiple girls all around, it’s not the lifestyle I wanted,” she told Ryan Seacrest.

The July cover of Playboy had already been sent to print, featuring Harris on the cover with the line, “America’s Princess. Introducing Mrs Crystal Hefner”.

Solution? Red stickers saying “Runaway Bride!” were slapped on the cover.

Harris auctioned her ring at Christies’s for half of its $90,000 value and told Howard Stern she only had sex with Hefner once.

It lasted “like, about two seconds,” she said.

“I’m not turned on by Hef, sorry.”

Still, the pair reunited and wed in 2012, with Hefner saying their “dramatic” 60-year age gap didn’t matter: “Whatever time I have left, we want to spend together.”

After Hefner’s death, celebrities including Kim Kardashian (“Paris & I are reminiscing about the Playboy parties at the mansion”) and Jenny McCarthy paid tribute to him.

Repeat covergirl Pamela Anderson posted a teary video and poem to Hefner on Instagram.

“People tell me all the time that I was your favourite,” she wrote.

“You had a piece of paper in your pocket you showed me – with my name Pamela with a heart around it.”

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