Cate Campbell has upstaged world champion sister Bronte to claim 100m freestyle gold at the Rio Olympic swimming trials in Adelaide on Tuesday night.

She clocked a world class 52.38 – just shy of her 52.33 PB – to relegate reigning world champion Bronte Campbell (52.38) to silver.

However, both locked in individual 100m berths to ensure they will contest the same event at Rio.

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Bronte Campbell only qualified for the 50m freestyle at her first Games at London in 2012.

Cate Campbell contested both the 100m and 50m events at London.

It marks the third Olympics for Cate Campbell, the 2013 world 100m champion.

They became the first siblings to make Australia’s Olympic swim team in four decades when they qualified for the London team.

Cate Campbell overcame a wrist complaint while her sister is nursing a shoulder injury to impress in Tuesday night’s 100m final.

Meanwhile, man of the moment Cameron McEvoy qualified fastest for Wednesday night’s 50m freestyle final.

James Magnussen kept alive hopes of an individual Rio berth after his 100m disappointment by being third fastest in the 50m semi-finals on Tuesday night.

World champion Mitch Larkin wins the 200m backstroke. Photo: AAP

Still buzzing from a record-breaking 100m effort, McEvoy topped the 50m semi-finals with 22.12 seconds ahead of Matt Abood (22.24) and Magnussen (22.34).

The 50m final is on Wednesday night.

A top-two finish in the final within the qualifying time will guarantee an individual Olympic nod for Rio.

McEvoy clocked the third-fastest 100m time ever to claim gold on Monday night.

It was just 0.13 of a second outside Brazil’s Cesar Cielo’s 2009 world record.

The one-lap “splash and dash” is Magnussen’s chance to earn an individual Rio berth after being left in McEvoy’s 100m wake.

Dual world champion Magnussen was fourth in the 100m final.

He is still in consideration for the 4x100m freestyle relay team for Rio.

Australia’s Rio swimming team is finalised on Thursday.

Meanwhile, world champion Mitch Larkin clocked the world’s fastest time of the year (one minute, 53.90 seconds) to claim 200m backstroke gold.

It would have won London Olympic bronze but it was short of his PB and Australian record 1:53.17.

Runner-up Josh Beaver also made the Rio team cut.

Commonwealth Games champion Taylor McKeown clocked a personal best 2:21.45 to win the 200m breaststroke gold.

Daniel Tranter (1:58.72) defended his 200m individual medley title but not in Rio qualifying time.

Top qualifier Thomas Fraser-Holmes withdrew from the final after already qualifying in three other Rio events.

World champion Emily Seebohm (2:08.10) is fastest qualifier for Wednesday night’s 200m backstroke final.

Grant Irvine (52.09) is fastest ahead of Wednesday night’s 100m butterfly final.

-AAP